Monday, February 23, 2015

Crossing the P-river: A complete bacterial genome assembled de novo using only nanopore sequencing data

 
 
We interrupt our regularly scheduled program, you probably remember this entry from fifteen days ago:
 Here is a new piece of that puzzle, with the right sensor (the Oxford Nanopre MinION instrument a long read sequencer), things that used to be difficult are enabling the flooding of a new tsunami: A complete bacterial genome assembled de novo using only nanopore sequencing data by Nicholas James Loman , Joshua Quick , Jared T Simpson

A method for de novo assembly of data from the Oxford Nanopore MinION instrument is presented which is able to reconstruct the sequence of an entire bacterial chromosome in a single contig. Initially, overlaps between nanopore reads are detected. Reads are then subjected to one or more rounds of error correction by a multiple alignment process employing partial order graphs. After correction, reads are assembled using the Celera assembler. We show that this method is able to assemble nanopore reads from Escherichia coli K-12 MG1655 into a single contig of length 4.6Mb permitting a full reconstruction of gene order. The resulting assembly has 98.4% nucleotide identity compared to the finished reference genome.
The software pipeline used to generate these assemblies is freely available online at https://github.com/jts/nanocorrect.
 
 
 
Related blog entries:

 

 

 
 
 
 
Join the CompressiveSensing subreddit or the Google+ Community and post there !
Liked this entry ? subscribe to Nuit Blanche's feed, there's more where that came from. You can also subscribe to Nuit Blanche by Email, explore the Big Picture in Compressive Sensing or the Matrix Factorization Jungle and join the conversations on compressive sensing, advanced matrix factorization and calibration issues on Linkedin.

No comments:

Printfriendly